Extraction of Tannins

Things to be needed:

500ml Erlenmeyer flask
Hot Place
Evaporating dish
Separatory funnel
Funnel (optional)
Filter paper
Beaker (optional)
Sample (powdered)
Diethyl Ether
Acetone (70%)

Procedure:

1. Accurately weighed 100 grams portioned of the powdered sample and subject to reflux for 15 minutes in a 500 ml Erlenmeyer flask with enough 70% acetone to cover up the whole powdered sample. The flask will be removed from the hot plate and will be filtered.  
2. In a another flask, a sufficient amount of 70% acetone is to add into the filter paper to wash off the residue.
3. The washed solution will be combined to the filtrate. The filtrate will be subjected to extraction method using diethyl ether, and this will be done in five times, until a complete separation of the diethyl ether and tannin, which is the upper layer and lower layer, respectively. 
4. The tannin has to be collected using a separatory funnel. The collected tannin will be evaporated until the residue is obtained. The residue will be final product and called the extracted tannin.  
5. To compute the percentage of tannin use this formula:  
        %tannin = Wt.of dried Tannin / Wt. of the powdered sample x 100%